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Glass-Polydimethysiloxane Hybrid Microthermostat for Restriction Enzyme Digestion

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Abstract:

This paper reports a low-cost microthermostat that is able to maintain a constant temperature necessary for restriction enzyme digestion. Polydimethylsiloxane (PDMS) and Pyrex glass were used to make the microthermostat, because PDMS is a cheap and mass-producible material and both PDMS and glass have very good biocompatibility compared to the more commonly used silicon. A heater made of Au wiring patterned on Pyrex glass was used to control the temperature. A PDMS replica molding technique was used to fabricate a reaction chamber with 3.6 μl capacity. Restriction enzyme digestion was performed by using the fabricated microthermostat and by a conventional method. Then, using gel electrophoresis, we compared results between the microthermostat and conventional methods. It was found that restriction enzyme digestion using the microthermostat required 5 min of heating.

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Periodical:

Materials Science Forum (Volumes 544-545)

Pages:

335-338

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.544-545.335

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Online since:

May 2007

Authors:

Yong Jun Ko, Dae Jin Kim, Woong Cho, Yoo Min Ahn, Seung Yong Hwang

Keywords:

DNA Chip Assay, Glass-PDMS Chip, Microthermostat, Restriction Enzyme Digestion

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] Z. Zhan, C. Dafu, Y. Zhongyao and W. Li, in: 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine & Biology, Lyon, France, (2000), p.25.

DOI: 10.1109/mmb.2000.893733

Google Scholar

[2] C.G.J. Schabmueller, A.G.R. Evans, A. Brunnschweiler, G. Ensell, D.L. Leslie and M.A. Lee, in: Proceedings of SPIE-The International Society for Optical Engineering, Vol. 4019, (2000), p.362.

Google Scholar

[3] I. Schneegaβ, R. Bräutigam and J.M. Köhler: Lab on a Chip Vol. 1 (2001), p.42.

Google Scholar

[4] Y.S. Shin, K. Cho, S.H. Lim, S. Chung, S. -J. Park, C. Chung, D.C. Han and J.K. Chang: J. Micromech. Microeng. Vol. 13 (2003), p.768.

Google Scholar

[5] R.H. Liu, J. Yang, R. Lenigk, J. Bonanno and P. Grodzinski: Anal. Chem. Vol. 76 (2004), p.1824.

Google Scholar

[6] M.A. Burns, B.N. Johnson, S.N. Brahmasandra, K. Handique, J.R. Webster, M. Krishnan, T.S. Sammarco, P.M. Man, D. Jones, D. Heldsinger, C.H. Mastrangelo and D.T. Burke: Science Vol. 282 (1998), p.484.

DOI: 10.1126/science.282.5388.484

Google Scholar

[7] T. Fujii: Microelectron. Eng. Vol. 61-62 (2002), p.907.

Google Scholar