Comparison of Gene-Expression of MC3T3-E1 Osteoblastic Cell Cultured on Machined Titanium Surface and Resorbable Blast Material Titanium Surface

S.H. Park; H.H. Kim; H.S. Lee; Y.S. Choi; A.R. Pae; M.R. Kim

doi:10.4028/www.scientific.net/KEM.361-363.1095

Paper Titles

Changes in Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocomposite Sponge under Pressure/Perfusion Culture
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Comparison of the Biological Behavior of Wollastonite Bioceramics Prepared from Synthetic and Natural Precursors
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Biological and Biomechanical Behavior of Titanium Implants with Fluoridated Hydroxyapatite Coatings
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Gene-Expression of MC3T3-E1 Osteoblast Cells Cultured on Anodized Titanium Surface and Machined Titanium Surface by Using cDNA Microarray
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Comparison of Gene-Expression of MC3T3-E1 Osteoblastic Cell Cultured on Machined Titanium Surface and Resorbable Blast Material Titanium Surface
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Proliferation of Osteoblast-Like Cells on Zirconia/Alumina Nanocomposite
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The Experimental Study of Culture of Osteoblast-Like Cells (MG-63) on Nanometer Magnetic Composite Substrate In Vitro
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Rat Bone Marrow Cell Responses on the Surface of Hydroxyapatite with Different Topography
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Preliminary Results of In Vitro Study of Cell Growth in a 45S5 Bioactive Glass as Bone Substitute Using Scanning Electron and Confocal Microscopies
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HomeKey Engineering MaterialsKey Engineering Materials Vols. 361-363Comparison of Gene-Expression of MC3T3-E1...

Comparison of Gene-Expression of MC3T3-E1 Osteoblastic Cell Cultured on Machined Titanium Surface and Resorbable Blast Material Titanium Surface

Abstract:

Because of its high biocompatibility, hydroxyapatite(HA) has been considered as a good blasting material. DNA microarray is a new molecular technology that enables the analysis of gene expression in parallel on a very large number of genes, spanning a significant fraction of the human genome. It is a qualitive analysis (e.g. it can differentiate each single gene) and quantitative, since it has the sensitivity to detect a change of expression level in the investigated cells when compared to normal samples. The aim of this study is to define the cell attachment and the genetic effect of machined surface implant and RBM (resorbable blast media) surface implant on the osteoblastic cell (MC3T3-E1 osteoblastic cell line) by cDNA microarray slide containing 21575 genes. Cells were cultured on machined grade 4 titanium disks(Group 1, machined surface) and disks of RBM (Group 2) and the samples were moved to new dishes and media were added and the plated disks were cultured for 24 hours. Total RNA extraction was performed with Qiagen mini kit (Qiagen, Chatsworth, CA, USA) for microarray assay. Microarray assay after culturing the cells on the machined surface and RBM surface revealed that osteoinductive molecules appeared more prominent on the RBM surface, whereas the adhesion molecules on the biomaterial were higher on the machined surface than RBM surface.