Paper Titles
Optimisation of the Aqueous Precipitation Synthesis of Silicate-Substituted Hydroxyapatite
p.55
Synthesis of Si-Containing Tricalcium Phosphate and its Sintering Behavior
p.59
Structure and In Vitro Solubility of Silicon-Substituted Hydroxyapatite
p.63
Synthesis and Phase Stability of Silicate-Substituted α-Tricalcium Phosphate
p.67
Protein Adsorption Properties of Hydrothermally Prepared Hydroxyapatite
p.71
Vibrational Properties of Sodium Substituted β-Tricalcium Phosphate
p.75
Exchange Reactions at Calcium Phosphates Surface and Applications to Biomaterials
p.79
Preparation of Spherical Porous Granules Composed of Rod-Shaped Hydroxyapatite and Evaluation of their Protein Adsorption Properties
p.83
Surface Structure, Hydration and Cationic Sites of Nanohydroxyapatite
p.87

Protein Adsorption Properties of Hydrothermally Prepared Hydroxyapatite

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Abstract:

Micrometer-sized hydroxyapatite (HA) with rod-shape was prepared to investigate protein adsorption onto its crystal face, through hydrothermal processing. Adsorption of Bovine serum albumin (BSA) and Lysozyme from chicken egg white (LSZ) on rod-shaped HA crystals with approximately 50 μm in length and 1 μm thickness was examined in phosphate buffer solution. The amount of adsorbed BSA was decreased with increasing phosphate concentration in the examined solution. Adsorption of LSZ was increased with increasing phosphate concentration in the buffer solution up to 50 mmol/L (mM), while it was remarkably decreased when the phosphate concentration reached approximately 100 mM. These phenomena lead to understanding of chromatographic characters of HA for separation of acidic and basic proteins.

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Periodical:

Key Engineering Materials (Volumes 361-363)

Pages:

71-74

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.361-363.71

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Online since:

November 2007

Authors:

Giichiro Kawachi, T. Watanabe, K. Kikukta, Chikara Ohtsuki

Keywords:

Crystal Morphology, Hydrothermal Method, Hydroxyapatite (HAP), Protein Adsorption

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© 2008 Trans Tech Publications Ltd. All Rights Reserved

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