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Specific Immobilization of Streptavidin on Mixed Self-Assembled Monolayers as Mixing Ratio

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Abstract:

We report on the distribution of mixed self-assembled monolayers (SAMs) composed of biotinylated and diluent alkylthiolates for streptavidin immobilization. Two thiol derivatives, 11-mercapto-1-undecanol (MUOH) and 11-mercaptoundecanoic-(8-biotinylamido-3,6-dioxaoctyl) amide (MBDA), were employed for mixed SAM. These thiols formed self-assembled monolayer without local domain, and streptavidins were immobilized onto biotinylated gold surface without nonspecific binding. In order to find the optimized condition of immobilization of streptavidin, we controlled the mixing ratio of two kind thiols by colorimetric detection assay, and the immobilization was characterized by atomic force microscopy (AFM), scanning tunneling microscopy (STM), and ellipsometer.

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Periodical:

Solid State Phenomena (Volumes 121-123)

Pages:

495-498

DOI:

https://doi.org/10.4028/www.scientific.net/SSP.121-123.495

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Online since:

March 2007

Authors:

Jun Hyung Park, Buyng Su Park, Gu Huh, Seung Hyun Lee, Hyun Sook Lee, Il Hoon Cho, Se Hwan Paek, Hai Won Lee

Keywords:

Atomic Force Microscope (AFM), Mixed Self-Assembled Monolayer, STM, Streptavidin Immobilization

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© 2007 Trans Tech Publications Ltd. All Rights Reserved

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