Based on the random vibration and system identification theory, the ambient vibration testing of two adjacent transmission towers were carried out, dynamic characteristics of the towers were obtained from ambient vibration data. The result shown that the suspended cables not only have mass effects, but have coupled stiffness effects applied to tower on transverse direction and longitudinal direction. At the same, testing value shows that the two adjacent towers have basically the same dynamic characteristics and damping ratio of the two transmission towers less than 0.01. Owing to the influence of conductors, frequency of the insulators is different to the towers at the two directions. It is disadvantage for wind-induced response of transmission line system under ambient loads.