Monitoring ground subsidence of roadbed in permafrost region is very important for stability evaluation and safety management. To test its applicability to deformation monitoring for railway subgrade settlement in plateau permafrost region, satellite differential interferometric synthetic aperture radar technology (D-InSAR) has been applied to detect subgrade deformation in one test site along the Qinghai-Tibet railway with gathered interferometric SAR images. The preliminary experimental results showed that satellite D-InSAR technology can effectively acquire long-time deformation sequence through analyzing the ground scatterers with relative stable phase and are in accordance with the results obtained by conventional in-situ ground leveling. Along the Qinghai-Tibet railway in permafrost regions, it is found that settlement is the main behavior of subgrade deformation and the deformation amount of railway bridge is less than the sliced or the crushed rock embankment. It has very important engineering significance for state key infrastructure long-term monitoring system in plateau permafrost region.