In order to ensure the prestress establishment of large-span suspendome, research on the measure-control methods for large-span suspendome during prestress construction process is performed, by summarizing the existing measure and test methods. According to engineering practice at present, the paper classifies the measure-control content of large-span suspendome during prestress construction period into two parts: internal force of cable-strut and deformation of steel structure. Taking practical project of a gymnasium as an example, the axial forces of hoop cables, radial steel tie rods and struts are measured, and also the verticality of struts. The test results are analyzed, and compared with the calculated value. The results show that, the internal forces of cable-strut and verticality of struts can both meet the design requirement, by mechanical analyzing and precise monitoring of the whole prestress construction process. The conclusion is that, before the prestress construction of large-span suspendome, construction mechanics analysis must be carried out in details to determine the control content and its target value, right measure-control methods are chosen, and then the effective establishment of prestress can be achieved.