Novel targets against HIV-1 are booming recently. APOBEC3G has the potential to inhibit the replication of HIV-1, while its antiviral activity is counteracted by Vif. The antiviral mechanism of APOBEC3G and the antagonistic mechanism of Vif have drawn great attention. Considering the occurrence of interaction between Vif and APOBEC3G should be based on that these two proteins coexist in the same cells, a eukaryotic bicistronic vector was adopted to express both Vif and APOBEC3G simultaneously. The expression and distribution of Vif and APOBEC3G proteins are detected with fluorescent microscope.