Paper Title:
Expression of HIV-1 Vif and Human APOBEC3G with a Bicistronic Vector
  Abstract

Novel targets against HIV-1 are booming recently. APOBEC3G has the potential to inhibit the replication of HIV-1, while its antiviral activity is counteracted by Vif. The antiviral mechanism of APOBEC3G and the antagonistic mechanism of Vif have drawn great attention. Considering the occurrence of interaction between Vif and APOBEC3G should be based on that these two proteins coexist in the same cells, a eukaryotic bicistronic vector was adopted to express both Vif and APOBEC3G simultaneously. The expression and distribution of Vif and APOBEC3G proteins are detected with fluorescent microscope.

  Info
Periodical
Chapter
Chapter 2:Materials Science and Bioengineering
Edited by
Barry Tan
Pages
141-145
DOI
10.4028/www.scientific.net/AMM.108.141
Citation
Y. S. Yang, X. N. Sun, D. Liu, X. L. Wang, S. S. Shen, Z. L. Li, Y. Zeng, "Expression of HIV-1 Vif and Human APOBEC3G with a Bicistronic Vector", Applied Mechanics and Materials, Vol. 108, pp. 141-145, 2012
Online since
October 2011
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Price
$32.00
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