Paper Title:
Separation and Purification of Alcaligenes Biodemulsifier by Dichloromethane-Water Extraction and Silica Gel Column Chromatograph
  Abstract

Biodemulsifier is one of the green demulsifier which could be hopeful used in crude oil industry. In this paper, pretreatment with dichloromethane and water extraction was used to extract active demulsification substance from the demulsifying bacteria Alcaligenes sp. S-XJ-1. The crude extract were consist of four amino compounds and one composition of saccharide by thin layer chromatography. It was established experiment condition of silica gel column chromatograph for crude extract purification. Demulsification ratio of the purified sample G4 was 70.8% after 48h. The purified active substance was a peptide compound by further analysed with infrared spectrum and amino acid analysis.

  Info
Periodical
Chapter
Chapter 2:Materials Science and Bioengineering
Edited by
Barry Tan
Pages
152-158
DOI
10.4028/www.scientific.net/AMM.108.152
Citation
X. F. Huang, K. M. Peng, L. J. Lu, J. Liu, "Separation and Purification of Alcaligenes Biodemulsifier by Dichloromethane-Water Extraction and Silica Gel Column Chromatograph", Applied Mechanics and Materials, Vol. 108, pp. 152-158, 2012
Online since
October 2011
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