Paper Title:
A Microfluidic Platform for Multi-Antigen Immunofluorescence Assays
  Abstract

Traditional cell-based assays such as cell immunoassay that utilizes plastic (chamber slides, dishes, microtiter plates), Magnetic bead, enzyme-linked immunsorbent assays (ELISA) [1], FACS cell sorting is labor intensive, time consuming, and requires a large numbers of cells or reagents. In this report, a microfluidic device integrated with cell culture, washing, fixation, and antigen-antibody reaction is presented for high-throughput immunoassay. Using this microfluidic device, each assay can be performed on a small number of cells and nanolitre or picolitre of reagents, this is particularly beneficial for rare or expensive cell types such as stem cells, or flow sorted cell populations. The capability of the microfluidic device was demonstrated for seeding human umbilical cord blood mesenchymal stem cells (UC-MSCs) in chambers and detecting the expression of surface markers (CD34, CD44, CD45, CD73, CD105, HLA-DR) by immunofluorescence assay.

  Info
Periodical
Chapter
Chapter 2:Materials Science and Bioengineering
Edited by
Barry Tan
Pages
200-205
DOI
10.4028/www.scientific.net/AMM.108.200
Citation
B. Y. Zhang, H. X. Song, T. Chen, Z. H. Wang, "A Microfluidic Platform for Multi-Antigen Immunofluorescence Assays", Applied Mechanics and Materials, Vol. 108, pp. 200-205, 2012
Online since
October 2011
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Price
$32.00
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