Taxus media is the natural hybrid variety of Taxus cuspidate and Taxus baccata, and has been used as an anticancer plant with some medical components-taxanes. In this study, ultra performance liquid chromatography was used for quantiﬁcation of three major bioactive taxanes in Taxus media, which were collected in Zhejiang province and Sichuan province (China). As a result of this study, a convenient, accurate, rapid and reproducible UPLC method has been developed to successfully quantify 10-Deacetylbaccatin Ⅲ (10-DABⅢ), Cephalomannine and Taxol in the needle and twig of Taxus media. The results indicate that the sequence of the contents of three active components in Taxus media with different parts was needle>twig; the sequence of the contents of three active components in Taxus media with different growth years was 5 year old >4 year old >3 year old; and there were great differences in the contents of three active components in Taxus media between Zhejiang province and Sichuan province. The results also provided a scientific basis for uses of Taxus media.