Nanoparticles usually prepared through chemical synthetic method. In our study, nanoCoenzyme Q10 was prepared by water jet comminution process. The results were characterized with scanning electron microscope (SEM) and the color change of the water solution. The color becomes lighter when the particles have a smaller size. The effect of comminution is related to the number of process cycles. nanoCoenzyme Q10 water solution (particle size less than 50nm) was obtained through water jet comminution at 50Mpa with five cycles. This new method not only solves the problem of physical comminution collection, but also gives an effective way to prepare natural nanoparticles.