Nowadays, various environmental regulations are being strengthened because of air pollution caused by exhaust gas emission of the automobiles. Biodiesel has been recognized as an alternative energy resource since it can be used without the modification of existing diesel engines and contains oxygen in itself, so the engine performance didn’t have large differences in comparison with the diesel fuel but remarkably reduces smoke emissions. The main objective of this study is to investigate usability of non-esterified biodiesel as an alternative fuel in a common rail direct injection diesel engine. The non-esterified biodiesel has not generated glycerin in esterificaion process and reduced the 20 percent of cost because it has not used methanol in the production process. Experiments were conducted by using the 5 percentage of biodiesel and 4 percentage of biodiesel with 1 percentage of WDP (water dipole power) in diesel fuel. Based on the experimental analysis the smoke emission of biodiesel was reduced significantly, but power, torque, and brake specific energy consumption was similar in comparison with commercial diesel fuel.