A vibration calculation model for a large-scale hydraulic turbine group is built to act on the unbalanced force between the rotor and the runner. Take the Francis turbine group for example. Using the finite element method, the units lateral vibration characteristics of rotating components were calculated and analyzed. The paper discusses the impact of location and the guide bearing stiffness on the lateral vibration characteristics of the unit. Based on the elastic supporting models, the paper also compares the results of the guide bearing stiffness modified simultaneously and respectively. Results show that the guide bearing stiffness has a significant impact on the lateral vibration characteristics of the location of the unit, which provides a reference for the reduction of vibration and the optimization of the supporting structure of the turbine unit.