Pd nanoparticles have been prepared on the surface of glassy carbon electrode (GCE) by electrochemical deposition method and applied for the nonenzymatic detection of H2O2. Scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) were employed to investigate the as-prepared Pd nanoparticles on the surface of GCE. The electrochemical properties of Pd nanoparticles modified GCE were also characterized by cyclic voltammetry and chronoamperometry. The results showed that Pd nanoparticles modified GCE had a favorable catalytic ability for the reduction of H2O2 in PBS medium (pH=7.6). At an applied potential of -0.06 V, the nonenzymatic H2O2 sensors produce high and reproducible sensitivity to H2O2 with 52.45 μA/mmol۰dm–3. Linear responses were obtained over a concentration range from 0.15 mmol۰dm–3 to 18 mmol۰dm–3 with a detection limit of 25 μmol۰dm–3 (S/N=3). Additionally, it exhibited a rapid response time (within 1s), which was much faster than some nonenzymatic H2O2 sensors.