The production rate and pellicle thickness of bacterial cellulose (BC) from Gluconacetobacter xylinus ATCC 23768 could be improved by Taguchi method. The initial pH is the important factor of medium to affect the BC production. After the confirmed experiment, the optimum modified YPD medium is: initial pH=4.5, glucose concentration = 2% (w/v), acetic acid concentration= 1.5% (v/v), medium volume = 430 mL and vessel basal area = 246 cm2. By the modified factors and levels, the maximum wet BC production rate and wet film thickness could be increased 53.1% to 111.90 g-BC/L and 43.3% to 4.43 mm, respectively. Higher basal area could get higher pellicle production rate.