The paper, based on the MIL-STD-1553B bus communication protocol, makes the computer-aided analysis to optimize bus transmission algorithm. An optimized algorithm is proposed by rearranging the commands set by the bus controller (BC), achieving the bus load balance in a major period. Through the command sequence optimization, this algorithm could manage the commands to be transmitted and generate an optimized bus command table. Based on the algorithm, software used in the PC is development, which could manage the commands to be transmitted, and generate optimized command table for bus communication transmission. The actual application shows that this algorithm can do well in optimizing the bus command table and improve the efficiency of bus transmission.