An electromechanical control system of a novel bioreactor for ligament tissue engineering, combined with culture medium perfusion control, cyclic mechanical loading and displacement coordinated control, strains and temperature measure and control, based on programmable logical controller (PLC), was designed and implemented. Well-controlled mechanical stimulations (resolution of <0.01mm for translational and <0.1° for rotational strains, cyclic loading frequency of up to 1 Hz) could be applied to the growing tissue, especially to the tissue engineered anterior cruciate ligaments (ACLs). The novel control system could complete detection and control functions of multi-dimensional mechanical strain in the same axis (resolution of 0.01N for tension and 0.01Nm for torsion strains). An online parameter measuring device of PO2 ，which did not consume oxygen and independent of flow rate, and were incorporated into the culture medium recirculation loop, was designed. The displacement and mechanical stimulate coordinated control strategies were achieved. The aim of this control system is to meet the functions of bioreactor for the ACLs tissue engineering in both research and clinical applications.