Paper Title:
Disodium Salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) Phenyl Phosphate as a Fluorescent Probe for Determination of ALP
  Abstract

A convenient method to synthesize disodium salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) phenyl phosphate (OXDP), a substrate for the fluorescent determination of alkaline phosphatase (ALP) activity, via an one-pot procedure of condensation between the benzoic acid and 2-hydroxybenzhydrazide at triethylamine alkaline solution in the presence of propylphosphonic anhydride, was realized. Preliminary tests reveal that the lessened fluorescence intensity resulting from enzyme reaction is proportional to ALP activity in the range of 1.0-25.0 U/L with the detection limit of 0.25U/L, which can be successfully applied to assess ALP in human serum with good precision.

  Info
Periodical
Chapter
Chapter 3: Chemical and Biomedical Engineering
Edited by
Honghua Tan
Pages
1109-1114
DOI
10.4028/www.scientific.net/AMM.138-139.1109
Citation
Y. J. Lei, O. Y. Jie, M. Ding, "Disodium Salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) Phenyl Phosphate as a Fluorescent Probe for Determination of ALP", Applied Mechanics and Materials, Vols. 138-139, pp. 1109-1114, 2012
Online since
November 2011
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Price
$32.00
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