Paper Title:
In Silicon Cloning and Bioinformatics Analysis of an Eukaryotic Initiation Factor 4E Gene from Grimmia pilifera
  Abstract

GH425 gene comes from the Grimmia pilifera drought stress of cDNA library.In this experiment,we have got the full sequence of GH425NO.1 by E-cloning which using GH425 as gene probe,in Physcomitrella patens DNA Datebase.Through using ORFfinder to find out the longest ORF and design primer for it,then, validated the Physcomitrella patens by PT-PCR,and we have obtained corresponding band and proved that the result of silicon cloning is correct and the fragment is contained in Grimmia pilifera P.Beauv.Now,we know the sequence encodes Eukaryotic initiation factor 4E by Blastx,and analysis it with Bioinformatics.

  Info
Periodical
Chapter
Chapter 3: Chemical and Biomedical Engineering
Edited by
Honghua Tan
Pages
1132-1138
DOI
10.4028/www.scientific.net/AMM.138-139.1132
Citation
W. Sha, L. Wu, X. H. Song, "In Silicon Cloning and Bioinformatics Analysis of an Eukaryotic Initiation Factor 4E Gene from Grimmia pilifera", Applied Mechanics and Materials, Vols. 138-139, pp. 1132-1138, 2012
Online since
November 2011
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$32.00
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