Fermentative hydrolysate of extruded corn gluten with higher solubility and antioxidative property by Bacillus natto was prepared. Extrusion of corn gluten was applied as pretreatment before fermentation. The best fermentative hydrolysis results can be obtained by fermenting at 34°C for cultivation time of 32 h, and flask rotation speed and initial pH of culture media were 200 r/min and 6.5-7.0 respectively. Soluble protein content and antioxidative activity of the fermentative hydrolysate reached 24.95 mg/ml and 259.21 U/ml respectively under the optimized culture condition. The molecular weight of the peptides in the optimal hydrolysate distributed mainly over 860-5300 Da determined by gel filtration chromatography. The hydrolysates displayed good solubility and antioxidative activity.