Paper Title:
Enhanced Production of Mycelia by the Medicinal Mushroom Cordyceps militaris Using Plackett-Burman Design and Response Surface Methodology
  Abstract

Response surface methodology (RSM) was used for statistical optimization of fermentation medium that influenced the yield of endo-polysaccharide from cultivated mycelia of Cordyceps militaris. First, the Plackett-Burman design was used to evaluate the effects of ten variables including glucose, maltose, peptone, yeast extract, KH2PO4, MgSO4, CaCl2, VB1, inoculum density and medium capacity. Among these variables, glucose, peptone and yeast extract were identified to have the significant effects. Subsequently, response surface methodology based on a five-level three-factor central composite design was employed to determine the maximum dry weight (DW) of mycelial biomass at optimum concentration of glucose, peptone and yeast extract. The mycelia growth was found to correlate to the three parameters that could be represented by second-order polynomial models. The optimal values of the three parameters were determined as 4.62% glucose, 3.36% peptone and 0.43% yeast extract. The prediction DW was 23.727g/L. The actual experimental results were in agreement with the prediction.

  Info
Periodical
Chapter
Chapter 3: Chemical and Biomedical Engineering
Edited by
Honghua Tan
Pages
1209-1214
DOI
10.4028/www.scientific.net/AMM.138-139.1209
Citation
X. Y. Liu, F. X. Meng, Y. B. Zhang, H. He, W. Han, W. Juan, L. R. Teng, "Enhanced Production of Mycelia by the Medicinal Mushroom Cordyceps militaris Using Plackett-Burman Design and Response Surface Methodology", Applied Mechanics and Materials, Vols. 138-139, pp. 1209-1214, 2012
Online since
November 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Nukrob Narkprasom, Tzou Chi Huang, Yuan Kuang Guu
Chapter 9: Biomaterials and Preparation Technology
Abstract:The quantitative effects of oxygen supply in terms of shaking speed and medium volume on the production of mycelia and extracellular...
975
Authors: Mei Lin Cui, Guo Qing He
Chapter 3: Sustainable Manufacturing Technologies
Abstract:In this paper, yeast extract concentration, incubation days and inoculation was made as independent variables, the production of...
259
Authors: Xiong Ya, Min Jie Li
Chapter 8: Medicine and Health Engineering, Biology Technology
Abstract:Termitomyces albuminosus is a kind of local distinctive wild edible fungi in southwest of China. It is delicious, rich in nutrition and...
810