Paper Title:
Comparison of Two Protein Extraction Methods for Tea Residues
  Abstract

Alcohol-alkali method and base digestion method were investigated to extract proteins from tea residues, respectively. According to single factorial experiments, results showed that the optimal extraction technology of alcohol-alkali method were pH 12, temperature of 80 °C, ethanol concentration of 60%, liquid-solid ratio of 40, 60 min, and the protein extraction rate reached 15.0%. And the optimal extract conditions of base digestion were pH 12, temperature of 80 °C, liquid-solid ratio of 50, 80 min, which made the protein yield reached 31.5%. Furthermore, alcohol-alkali method was more beneficial to protein extraction from tea residues under lower temperature and weak alkali condition (40-60 °C, pH 8-10). While base digestion had higher extraction yield under high temperature and strong alkali condition (60-80 °C, pH 11-12).

  Info
Periodical
Chapter
Chapter 3: Chemical and Biomedical Engineering
Edited by
Honghua Tan
Pages
933-936
DOI
10.4028/www.scientific.net/AMM.138-139.933
Citation
X. Chen, H. Y. Luo, J. Yu, P. X. Yue, L. Zhou, Y. Ren, Y. H. Wang, W. P. Fang, X. H. Li, "Comparison of Two Protein Extraction Methods for Tea Residues", Applied Mechanics and Materials, Vols. 138-139, pp. 933-936, 2012
Online since
November 2011
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$32.00
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