It is widely acknowledged that image thinking is significant in medical education. The colorful pictures and animations need to be presented for the users (observers) to make the content easier to be understood. By the appropriate matching of pictures and text, readers are able to associate the text with the image on the screen to reach better understanding. In this case, 3d and dynamic images will take the place of the plane and static ones. The study software applied the image processing software and animation software, such as Photoshop, Flash software. 3d max software is used for processing and preparation the simulation stereo skeleton. the Java language of Cult 3D models help give the characteristics of model rotating and zooming in and out; Using multimedia interactive software, Neobook, makes sure interactive amity of interface and establishes a good interactive operating environment between the users and 3d models. For the basic medical education which has higher visual thinking demand, multimedia education has already be widespreaded and used. This software has interactive function and three-dimensional content, 3d instead of static, dynamic replace static state. It is not only keeping the traditional advantage (such as the logical arrangement of content, strictly text description, etc.), but also providing the learning reference material to the users which has intuitive visual experience, independently and freely self-selection, flexible processing. It is beneficial to understand and learn related contents. This study software shows rich and colorful pictures, 3d dynamic display contents, animation process of image to the users, so as to increase understanding and grasping the contents on human spine. It has multiple functions such as help studying and teaching.