Paper Title:
Improved Identification of White Blood Cells and Renal Tubular Epithelial Cells in Urine Using Cytology
  Abstract

Objectives: Conventional automatic identification system to differentiate white blood cells from renal tubular epithelial cells was limited by overlapping parameters and investigation of clear classification of these two cells could be critical to diagnosis and prognosis. Methods: Urine samples from 120 individuals (30 bladder cystitis, 30 glomerular nephritis, 30 pyelonephritis and 30 nephrotic syndrome) were collected. Urine sediments were stained by Sternheimer method and examined by TJYDSXG-1 microscopic cell analysis system including cell size, degree of cytoplasmic staining and nuclear coefficient of variation (CV). Peroxidase chemical staining was also employed to differentiate white blood cells (WBC) and renal tubular epithelial cells (RTEC) in sediments. Results: WBC in urine sediment was (8-13) μm, while (10-16) μm for RTEC, with 36% overlapping of nuclear CV. Peroxidase chemical staining intensity index is 0-4 for WBC and 0-1 for RTEC. Conclusions: Percentage of overlap between WBC and RTEC can be reduced to 7%-13% when Sternheimer staining was combined with peroxidase staining.

  Info
Periodical
Edited by
Robert Zhu
Pages
152-156
DOI
10.4028/www.scientific.net/AMM.140.152
Citation
X. G. Sun, D. J. Wei, X. C. Liu, Y. Z. Ma, X. Fan, K. S. Hu, "Improved Identification of White Blood Cells and Renal Tubular Epithelial Cells in Urine Using Cytology", Applied Mechanics and Materials, Vol. 140, pp. 152-156, 2012
Online since
November 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Akihiro Furuhashi, Yasunori Ayukawa, Ikiru Atsuta, Yunia Dwi Rakhmatia, Noriyuki Yasunami, Kiyoshi Koyano
X. Metals
Abstract:At the neck area of dental implant surface, machined surface (Ms) has been employed to avoid surface contamination. Recently, implants which...
559