Paper Title:
In Vitro Isolation of Intestinal Side Population Cells from Newborn Mice
  Abstract

To isolate side population cells from newborn mice small intestinal mucosa, and to investigate the feasibility of constructing the murine intestinal stem cell population by fluorescence activated cell sorting. Small intestine mucosa organoids of mice were isolated and dissociated into single cells. The side population cells were stained with Hoechst 33342 and propidium iodide, then sorted using fluorescence activated cell sorting. Total RNA and protein were purified from sorted fractions to detect Musashi-1 expressions by RT-PCR and Western-blotting. Single cell suspension from mouse small intestine mucosa contained a viable population of cells, which showed the side population phenotype and were sensitive to verapamil. These cells were enriched for Musashi-1 mRNA and MSI-1 protein expression. Results demonstrated that the side population fraction separated from mice intestinal mucosa is enriched for intestinal stem cells, the murine intestinal stem cell population can be successfully constructed with fluorescence activated cell sorting.

  Info
Periodical
Edited by
Robert Zhu
Pages
157-161
DOI
10.4028/www.scientific.net/AMM.140.157
Citation
Y. F. Zhang, J. Yang, Y. G. Tan, Y. H. Li, M. Yang, "In Vitro Isolation of Intestinal Side Population Cells from Newborn Mice", Applied Mechanics and Materials, Vol. 140, pp. 157-161, 2012
Online since
November 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Young Joo Jang, Young Sook Kil, Jee Hee Ahn, Jae Hoon Ji, Jong Seok Lim, M. Won, Hyang Sook Yoo
Abstract:The fission yeast, Schizosaccharomyces pombe is a single-celled free-living fungus that shares many features with cells of more complicated...
1
Authors: Jun Zhang, Ying Zhang, Ya Jun Duan, Lai Liang Ou, Yao Ting Yu, Deling Kong
Abstract:Recent study shows that endothelial progenitor cells (EPCs) and gene therapy technologies are effective strategies in the inhibition of...
31
Authors: Leandra S. Baptista, Carolina S.G. Pedrosa, Karina R. da Silva, Ronaldo J.F.C do Amaral, Michele C.L. Kochem, Hélio Menezes, Aris Sterodimas, José de Faria, Marcio R.R. de Oliveira, Radovan Borojevic
Abstract:Silks are naturally occurring polymers and fibroin, its filament core protein, has been shown to support stem cell differentiation in vitro,...
437
Authors: Yu Ping Li, Hu Su, Xiao Fang Pi, Yan Chun Gong, Xiang Yuan Xiong, Guang Jie Wu, Zi Ling Li
Chapter 10: Chemical Biology and Medicinal Chemistry
Abstract:The medicinal plant Scutellaria baicalensis Georgi has been used widely in traditional Chinese medicine for anti-inflammation, anticancer,...
1673
Authors: Wei Chung Liu, Chang Shu Tsai, Ya Yun Chen, Nien Tzu Keng
Chapter 2: Advanced Bio and Medical Engineering, Applications of Bioinformatics and Genetic Engineering
Abstract:Silica containing materials are often applied in bone tissue engineering, which may contact with bone marrow cells. However, the biological...
494