Paper Title:
Preparation, Partial Isolation of Antifreeze Peptides from Fish Gelatin with Hypothermia Protection Activity
  Abstract

Antifreeze proteins have characteristics of inhibiting the growth of crystals, decreasing the injury of cells and can retain the structure, texture and quality of productions. The purpose of this study is to obtain natural antifreeze peptides, and to investigate the hypothermia protection activity on bacteria. Gelatin derived from shark skin was hydrolysed to obtain antifreeze peptides. The most appropriate protease and hydrolysis time was selected with the index of the hypothermia protection activity on bacteria. The hydrolysate was subsequently added on to Sephadex G-50 gel filtration column and SP-Sephadex C-25 column to acquire high activity fractions. The fraction of cationic peptides termed P2 shows higher antifreeze activity. The hypothermia protection assay shows that the survival rate of E.coli was 80.8 % when the concentration of peptides complexes was up to 500 μg/mL.

  Info
Periodical
Edited by
Robert Zhu
Pages
411-415
DOI
10.4028/www.scientific.net/AMM.140.411
Citation
S. Y. Wang, J. Zhao, Z. B. Xu, J. H. Wu, "Preparation, Partial Isolation of Antifreeze Peptides from Fish Gelatin with Hypothermia Protection Activity", Applied Mechanics and Materials, Vol. 140, pp. 411-415, 2012
Online since
November 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Jian Feng Qian
Chapter 10: Environmentally Sustainable Manufacturing Processes and Systems
Abstract:Urban sewage is a renewable energy source in the fields of heating and air conditioning engineering, and urban sewage source heat pump system...
3235
Authors: Kanageswary Sockalingam, Maizlinda Izwana Idris, Hasan Zuhudi Abdullah
Chapter 2: Materials and Technologies in Environmental Engineering, Bio- and Green Production
Abstract:Black tilapia (Oreochromis mossambicus) fish scales are good sources of gelatin. Fish scales needs to undergo adequate...
276
Authors: Ming Fu, Hong Yong Li, Yue Wang, Lin Fan, Dong Chen, Zhao Feng
Chapter 2: Nano and Inorganic Functional Materials
Abstract:High-performance ultrafine silver powder used as crystalline silicon solar cell front conductive paste was prepared by chemical liquid-phase...
378