In a dynamic environment of e-commerce, different business entities expect to interact with each other and have their transaction time include its cost minimized. In this paper, the concept of agent and multi-agent, as well as their structure, were introduced. At the same time, the negotiation protocol, negotiation strategies and the negotiation mechanism were also researched for e-commerce negotiation. The agent technology was exploited, based on which, the negotiation system was developed. This platform would improve negotiation efficiency by saving time and cost for e-commerce.