In this paper the effect of delamination position on the critical buckling load and buckling mode of hybrid composite beams is investigated. Experimental and numerical studies are carried out to determine the buckling load of delaminated composite beams. The laminated composite beams with various laminate designs of [G90]6, [C90]8, [C0/G0]4 and [C90/G90]4 were manufactured and tested to find the critical buckling load. Three different defect positions were placed through the thickness to find three main buckling modes. It was found that delamination position and lay-up can affect the buckling mode and also the critical buckling load. By approaching the delamination position to the outer surface of the specimen the buckling load decreases. The buckling process of hybrid and non-hybrid composite beams was also simulated by finite element software ANSYS and the critical buckling loads were verified with the relevant experimental results.