In order to verify the fitting degree of whole vehicle simulation modeling and practical vibration, the transportation truck subsystem simulation models are established and the whole vehicle virtual model is assembled with ADAMS software based on multi-body dynamics theory and virtual prototyping. The documents of rural road are created in the MATLAB with harmonic superposition principle according to the description of pavements in different grades in the national standard. Through loading road documents, the obtained vibration spectrums are compared with the measured spectrums. The RMS(Root of Mean Square) of the virtual test inosculates basically with the RMS of practical running-in test in each test point by analyzing. It shows that the simulation experimental results have enough reliability and the established virtual prototyping can be used as a basis for structural design of vibration simulation system.