Effects of fiber fabric type, glue spread and adhesive kind on stiffness of fiber-wood bonding interface were investigated. Four different kinds of fiber fabric were bonded into poplar sample with three different adhesives and three different glue spread. Shear modulus of samples were measured by compression shear test to represent the stiffness of bonding interface. The results indicated that stiffness of GFF (Glass Fiber Fabric)-wood and GFMa (Glass Fiber Mat)-wood interfaces was better than that of two other interfaces and shear modulus of CFF (Carbon Fiber Fabric)-wood samples were the least of all. And the effects of glue spread and adhesive kind on stiffness of fiber-wood interface were significant.