Motorcycle Ride Comfort is the important performance of motorcycle. In this paper, a rigid-flexible coupling virtual prototype is developed with CATIA, ADAMS and NASTRAN tools. Combined with motorcycle road simulator, the simulation platform of motorcycle ride comfort is built. Then with the input of pulse road, motorcycle ride comfort is simulated, and the simulation results are verified by experiment results. It indicates that the simulation results have a high credibility and the road simulator provides a good method of verification for simulation of motorcycle ride comfort.