The rapid development of tissue engineering brings new hope to the neurological function recovery after spinal cord injury. The three-dimensional structure of scaffold is a key factor to treat spinal cord injury in tissue engineering method. In this paper, low-temperature deposition manufacturing (LDM) is discussed and basic process about scaffold forming is brief related. The principle of low-temperature deposition manufacturing and some important process parameters were studied. Some experience values are obtained from experiment. The biological environment in vivo was proposed as an important basis for the design of spinal cord scaffold. Then the spinal cord scaffold is separated into gray matter and white matter induced function area by the separate layer of low porosity and small pore size. The experiment results show that the big pores of scaffold are round and regular. The scaffold includes a large number of irregular pore structure, and good penetrating. The scaffold porosity of 86.38% could well meet the needs of the high porosity.