With the rapid development of economic society, more and more people are suffering from the huge pressure that comes from all aspects. So many kinds of diseases have higher morbidity than before. Among these diseases gallstone is paid more attention because of its generality. To design a new type instrument that can dissolve cholesterin gallstone directly through peristaltic pumps controlled by industry compute and high speed Analog/Digital (A/D) and Digital/Analog (D/A) card. The instrument can regulate the amount of solvent automatically in order to adapt to the cholecyst according to the treatment parameter given by manipulator and the pressure of patients' cholecyst. Meanwhile, the date can be displayed on the screen. The working principle, structure design, hardware and software design have been presented in this paper. The instrument showed a good result both in vitro and in vivo for animals. These tests confirm that the instrument can enhance the efficiency of manual infusing to dissolve a stone and decrease the patients’ pain and sufferings, especially have more significance to the patients who aren't fit for operation and anesthesia. It has the advantages of quick treatment and micro hurt, fitting for clinic use. The new type instrument that can dissolve cholesterin gallstone is a kind of means that can be widespread used with micro hurt.