Elastic modulus is one of the most important performance parameters of wood-based material, and plays an important role for predicting and evaluating quality of wood-based material. At present, the traditional problems for measuring elastic modulus of wood-based material are: expensive testing instrument, short precision, low immunity to noise and less ease to connect. This paper presents a new elastic modulus-measuring system based on virtual instrument. The system ensures the following functions: (1) real-time data acquisition, (2) signal processing and analyze, (3) saving the file in the appropriate format, (4) computing dynamic elastic modulus by the natural frequency. The analytical results of our experiments show that it is feasible, reliable and effective to use the proposed instrumentation for measuring elastic modulus of wood-based floor.