We aim to combine the technology of capturing human motion and the technology of virtual reality to carry on assessment of human factors. The unique point in this method is that not only the reliable data of the maintenance worker can be gained, but also the quantitative analytic result based on the virtual environment can be obtained. In the paper, human motion capture technology, ergonomics evaluation and the interface technology have been considered comprehensively. overall technical program of human factors evaluation, which is based on human motion capturing technology, have been carried on; the technology, which include the captured data of human motion translating into the virtual environment, building the virtual human model and virtual human simulation, both based on captured data in the working site, are taken as innovations; replicable technology of the captured data in the virtual environment have been broken through. Carrying on the quantitative analysis of worker working postures, fatigue and human force and torque in the maintenance process, which is based on the technology of human factors evaluation by using the captured data in the working site, is researched. We have verified the feasibility of this technology through an example. The method provides a new way and operational technology for human factors assessment in maintenance process of aviation equipment.