A bisphenol-A glycidol ether epoxy resin was toughened in cryogenic temperature using reactive macroglycol as modifiers. The properties of modified epoxy resin were characterized by SEM and DMA. Phase-separated structure formed during curing process in the PPG and PTMG modified epoxy resins system, and did not occurred in the PEO modified epoxy resins system. The impact strength of epoxy resin increased at both room temperature (RT) and cryogenic temperature (CT, 77 K) using PEO as modifier. The DMA results confirm that the introduction of PEO chains in the structure of the epoxy increases the mobility of the molecular segment of epoxy network at both RT and CT.