The paper analyses CO2 trans-critical two stage compression refrigeration cycle with vortex tube expansion by thermodynamics method. And compare with CO2 trans-critical two stage compression refrigeration cycle with expansion value. The results show that in the calculated conditions of the paper, the performance of the cycle with vortex tube improves 2.4%~16.3% than the cycle with expansion value. The optimal discharge pressure maximizing COP of the cycle with vortex tube exists. With lower evaporating temperature or higher gas cooler exit temperature, COP of system decreases and COP improvement increases. The effect of cold fluid mass fraction on COP is not significant, but COP improvement increases more quickly with cold gas mass fraction increasing.