Paper Title:
Design and Performance of a High-Efficiency Cell-Electrofusion Device
  Abstract

A microfluidic cell-electrofusion device was developed for high fusion efficiency and automatic manipulation. It included an experimental platform, an electric signal generator and a microchip. Microstructure and controlling signal were elaborately chosen in order to realize precise cell manipulation, alignment and electrofusion. Compared with the traditional cell-electrofusion device, this device has some outstanding advantages. For example, short distance between two counter-microelectrodes will reduce the required voltage. A large number of microelectrodes can be fabricated on one small chip for high-throughput fusion. Transparent chip architecture benefited for microscopic observation and real-time recording. The integration of the experimental platform, signal generator and microchip is helpful for developing a portable cell-fusion system. On-chip electrofusion experiments of cucumber mesophyll protoplasts have been carried out and up to 40% fusion efficiency was obtained.

  Info
Periodical
Edited by
Zhou Mark
Pages
664-667
DOI
10.4028/www.scientific.net/AMM.52-54.664
Citation
J. Yang, J. Yang, Q. H. You, N. Hu, Y. Li, J. Chen, T. Y. Li, J. Xu, Y. Cao, "Design and Performance of a High-Efficiency Cell-Electrofusion Device", Applied Mechanics and Materials, Vols. 52-54, pp. 664-667, 2011
Online since
March 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Yong Chao Gao, Bai Tong Zhao, Wen Xiu Gao
Abstract:In this paper, we should analyze the characteristic of the solar grade poly-silicon up-graded by physical process and make comparison with...
119
Authors: Wei Zhang, Bo Zhang, Yu Long Zhang, Da Han, Yong Liang Zhou
Chapter 11: Biomaterials
Abstract:A selectively yeast cell trapping and arraying method was presented, utilizing PDMS micropillar array combining with chemical adhesion. Yeast...
2096
Authors: Jing Yi Chang, Yean Der Kuan, Chih Hsun Yao, Sheng Ching Chan
Chapter 1: Green Building
Abstract:During the average fifty or sixty years of building lifecycles, large amounts of energies are consumed at all stages, from the production of...
51
Authors: Okihisa Kobayashi, Yusuke Umezawa, Syoji Kanai, Masaru Hakoda
Chapter 2: Chemical Science and Technology
Abstract:In order to clarify the relation between ReKω and the electrical property of each intracellular part, we loaded temperature stress on the...
93
Authors: Aleksandr N. Aleinik, Marat R. Muhamedov, Natalia D. Turgunova, Rinat.G. Babaev, Natalia I. Karpovich
Chapter 4: Radiation Technologies in Medicine
Abstract:Radiation therapy is an organ sparing technique. However, radiation therapy near the liver, kidneys and other organs is not always possible...
381