Experiments were conducted in a horizontal multiphase flow test loop (50mm inner diameter, 40m long) to study the flow patterns for oil-gas-water three-phase flow and the pressure gradient fluctuation based on flow patterns. Using new methods of definition, 12 types of flow patterns were obtained and phase distribution characteristics of each pattern were analyzed. A new flow pattern (SW║IN) was firstly found in this work. Characteristics of the pressure gradient based on 7 flow patterns were carefully discussed. It was found that the pressure gradient increased with the increase of gas superficial velocity and oil-water mixture velocity. However, characteristics of the pressure gradient became complex with the increase of input water cut. The influence of flow structure of oil-water two-phase should be fully considered.