Rut disease and anti-rut performance of a 13km very long uphill expressway was studied, which indicates that standard rut testing methods and dynamic stability controlling standard values are not suitable for the very long uphill expressway. According to the study, the 52km very long continuous uphill expressway was divided into three sections based on the temperature and longitudinal slope condition. Many asphalt surface course combination structures were set for the three sections. Total-thickness rut tests were carried out for these combination structures at heavy loading, low speed and different temperature conditions with the developed Wheel Tracking Test equipment. A dynamic stability controlling standard value for the whole asphalt surface course of the 52km long uphill expressway was determined, and according to the dynamic stability controlling standard value, the optimal asphalt surface course combination structures were recommended for the three sections.