Waste newspaper fiber was filled in the collagen cross-linked by glutaraldehyde. The effects of deinking fiber, glycerol dosage, glutaraldehyde dosage on the mechanical properties of composite were investigated. It is found that the deinking treatment increases the strength of the composite material. The composite has better mechanical properties under the preparing conditions as follows: waste newspaper fiber content 10%, glutaraldehyde dosage12.5%, glycerol dosage 12.5%, pH 9.7, 60°C for 2h. The tensile strength and elastic modulus are 4.98Mpa and 0.09Gpa, respectively.