Present study deals with the evaluation of biosorptive removal of nickel by Flavobacterium sp. Experiments have been carried out to find the effect of various parameters such as initial pH, contact time and initial metal ion concentration. Adsorption equilibrium studies showed that Ni(II) adsorption data followed the Langmuir model, the maximum binding capacity of Ni(II) was 64.20 mg/g at pH 7.0. Kinetics of nickel biosorption by Flavobacterium sp. biomass is better described by pseudo second order kinetic model. The equilibrium isotherm data are very well represented by Langmuir isotherm equation, which confirmed the monolayer coverage of nickel onto Flavobacterium sp. biomass. It was also clearly observed that The present study indicated that Flavobacterium sp.biomass may be used as an inexpensive and effective biosorbent for the removal of Ni(II) ions from environmental wastewater.