Paper Title:
Establishing a Assay for Detection of Nonylphenol Estrogenic Effects
  Abstract

Environmental estrogen could mimic natural estrogens thereby disrupting the endocrine systems of human and animals. The actions of such endocrine disruptors have been studied mainly on reproduction and development. To explore the estrogenic effects of NP by reporter genebased assays we developed. pERE-GFP plamid was generated by inserting estrogen response element fragment into pGADD153-GFP. the recombinant was confirmed by restriction enzyme map and transfected into SPC-A1 cells to ensure the expression of green fluorescent protein.The assay we established in usful, NP could induce the estrogenic activities at any of the tested concentrations.

  Info
Periodical
Chapter
Chapter 5: Frontiers of Environment Engineering and Materials
Edited by
Dongye Sun, Wen-Pei Sung and Ran Chen
Pages
3003-3006
DOI
10.4028/www.scientific.net/AMM.71-78.3003
Citation
Y. Q. Liu, X. B. Lu, "Establishing a Assay for Detection of Nonylphenol Estrogenic Effects", Applied Mechanics and Materials, Vols. 71-78, pp. 3003-3006, 2011
Online since
July 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Yuan Song Zhang, Tian Fu Zhao, Ai Chun Zhao, Masao Nakagaki
Chapter 5: Structure, Properties and Processes of Textile Materials
Abstract:Silkworm BmN4 cells were transfected with the helper plasmid and the piggyBac vector( piggyBac-FLAG) in which was inserted with the spider...
779
Authors: Xin Yu Sui, Xiang Ru Liang, Yu Fu Hu, Guo Qing Shi
Chapter 17: Plant Protection and Biotechnology
5586
Authors: A. Apirakaramwong, Perayot Pamonsinlapatham, S. Techaarpornkul, Praneet Opanasopit, Suwannee Panomsuk, S. Soksawatmaekhin
III. Applications of Biomaterial
Abstract:Chitosan (CS) has a high potential for gene delivery into mammalian cells. However, its uptake mechanism is not well clarified. We...
485
Authors: Feng Hao, Zhong Hai Yuan, Zhi Xin Wang, Hui Jing Xu, Fang Fang, Xin Gang Guan, Jiang Yong, Li Yan
Chapter 10: Chemical Biology and Medicinal Chemistry
Abstract:Calcium-activated chloride channels (CaCCs) play pivotal roles in many physiological Activities, including transepithelial fluid secretion,...
1734
Authors: Wei Chung Liu, Chang Shu Tsai, Ya Yun Chen, Nien Tzu Keng
Chapter 2: Advanced Bio and Medical Engineering, Applications of Bioinformatics and Genetic Engineering
Abstract:Silica containing materials are often applied in bone tissue engineering, which may contact with bone marrow cells. However, the biological...
494