Paper Title:
Microwave-Assisted Extraction and In Vitro Antioxidant Evaluation of Polysaccharides from Enteromorpha prolifera
  Abstract

An efficient microwave-assisted extraction (MAE) technique was developed to extract polysaccharides from Enteromorpha prolifera (PEP). The operating parameters, such as microwave power, liquid/material ratio, temperature and extraction time, were optimized using orthogonal array design coupled with single factor method. PEP yield was determined by the phenol-sulfuric acid method. The optimum extraction conditions were determined as follows: microwave power, 700 W; liquid/material ratio, 40:1 (mL/g); temperature, 70 °C; and extraction time, 25 min. Under such conditions, PEP yield reached to 10.79 %. In comparison with conventional hot water extraction and ultrasonic-assisted extraction, MAE showed obvious advantages in terms of high extraction efficiency, saving energy, rapidity, solvent consumption, and so on. The data demonstrated that MAE could be a fast and reliable method for quantitative analysis of PEP. The scavenging capability of PEP to DPPH/hydroxyl radical reached to 65.2 % and 41.2 % at the concentration of 0.5 mg/mL. The reducing power of PEP was 0.354. Compared with butylated hydroxytoluene (BHT) and gallic acid (GA), the experimental results showed that DPPH radical scavenging activity of PEP was higher than that of BHT. Thus, PEP had good potential as a natural antioxidant used in functional food or medicine industries.

  Info
Periodical
Edited by
Yiyi Zhouzhou and Qi Luo
Pages
204-209
DOI
10.4028/www.scientific.net/AMM.79.204
Citation
B. Wang, G. Z. Tong, Y. L. Qu, L. Li, "Microwave-Assisted Extraction and In Vitro Antioxidant Evaluation of Polysaccharides from Enteromorpha prolifera", Applied Mechanics and Materials, Vol. 79, pp. 204-209, 2011
Online since
July 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Xi Hong Li, Chang Liu, Ya Jun Sun, Jin Du, Ya Ge Xing, Yao Tang
Abstract:Effects of lycopene on the oxidative stability of soybean oils were studied by adding different concentrations of lycopene [0.01, 0.02 and...
2303
Authors: Hong Fang Ji, Ling Wen Zhang, Hai Yan Zhang, Hai Juan Nan, Qing Wang, Hui Rong Zhang
Abstract:Cold water extracts from Hohenbuehelia serotina and Armillaria mellea fruit bodies were prepared and their antioxidant...
2840
Authors: Hong Fang Ji, Ling Wen Zhang, Hai Yan Zhang, Hai Juan Nan, Da Guang Yang, Ming Duo Yang
Session 2: Materials for Biomedical Engineering Application
Abstract:Hot water extracts from Armillaria mellea and Hohenbuehelia serotina fruit bodies were prepared and investigated for their antioxidant...
463
Authors: Ting Qiu, Bi Yu Wang, Xiao Cui Lin, Yan Xiang Wu
Chapter 9: Extraction and Separation
Abstract:This study aimed at evaluating the antioxidative activity of crude hsian-tsao extracted by acetone and the Fraction E purified by AB-8...
1784
Authors: Shi Rong Tang, Hui Song, Ying Jia Chen, Chun Hua Zhu
Chapter 4: Clean Production and Green Chemical Technology
Abstract:Synergistic extraction technology of ginger polyphenols and antioxidant activities were studied. The extraction yield of total polyphenols...
580