The aim of this paper was to analyze the biomethanization process of cattle manure with four different total solid percentages (15%, 20%, 25%, 30% TS) and three different stirring frequency. The experimental procedure was programmed to select the initial performance parameter and the operational parameter in a lab-reactor. The values of VFAs indicated that all the reactors showed no destabilization and at the end of the experiment the VFAs were consumed completely. The best performance for cattle manure biodegradation and methane generation was the reactor with 20% TS, with the biogas yield of 0.22 L/g VS and the methane yield of 0.11 LCH4/g VS. Furthermore, the better operational parameter of stirring frequency was stirring once every two days.