Purpose: A multi-leaf collimator leaf sequencing comparison program in the sense that it translates beam intensity maps into the least number of MLC field segments was presented. Methods: The IMRT leaf sequencing calculation program based on Galvin, Bortfeld algorithms was constructed. The output of the leaf sequencing program were the number of segment and the total number of monitor unit. Results: Assuming 15 x 15 bixel fields with an average of 10 intensity levels, Bortfeld algorithm could yield better result on example 1 while Galvin algorithm yielded better results on example 2. Conclusions: This represented a useful tool for optimizing the leaf sequencing of static multi-leaf collimator that can yield shorter treatment time and higher utilization of photons by comparing with the total number of monitor units and number of segments of existed typical algorithms. It was an effective strategy and could be applied in commercial Treatment Planning Systems.