The different age pleioblastus amarus particle were taken as the raw materials, and the wet-process bamboo powder-boards were made from the laccase-treated powder by hot pressing. The raw material chemical constituents were analyzed. The reactive oxygen species free radicals produced from the laccase-treated bamboo were detected by electron spin resonance. The physical and mechanical properties of the bamboo particleboards were measured. In order to provide some theoretical basis for producing bamboo particleboard by laccase activation, the relations between physical and mechanical properties of bamboo particle boards and chemical constituents, the reactive oxygen species free radicals were researched.