A method based on the 3D laser scanning is proposed for obtaining and analyzing the windshield's deformation in vehicle-pedestrian accident. With an actual traffic accident of vehicle-pedestrian collision taken for example, point cloud data of windshield’s deformation is scanned by EXAScan 3D laser scanner and the deformed 3D NURBS surface is obtained after fitting process. Based on the discussion about characteristics of curved surface of the vehicle’s front windshield and the principle of continuous curvature, this paper conducts a surface fitting with cubic non-uniform spline according to geometric information of grid surface around the deformation zone, and obtains the pre-deformed surface of windshield by sampling with spline curve. Compared with the pre-deformed surface of vehicle’s windshield, the maximum deformation depth is 48.509mm and its area is 0.3816m2 on the right side, while the maximum deformation depth is 36.341mm and its area is 0.2529m2 on the left side. The application results in actual traffic accident show that this method can be used to obtain 3D deformation information of overall impact area, which provide a new method for accurately inspecting the windshield’s deformation in vehicle-pedestrian accident. This method also provides a new research idea for quantitative saving the evidence in permanent, and it also has a great value in accident analysis and reconstruction.