A procedure for the dynamic simulation analysis of the globoidal indexing cam mechanism is presented. Fist of all, the 3D model of globoidal indexing cam mechanism is established. Then, the virtual model needed by ADAMS is directly achieved by converting the 3D assembly model through the interface of SimDesigner in CATIA. In ADAMS, the tendency curves of angle velocity, acceleration of the indexing plate and contact forces between the roller and cam are made by dynamic simulation. It is very obvious to see the influence of the thickness of preload plate and assembly error on the maximum acceleration of indexing disk from the simulation result, and it will contribute to the design and assembly the globoidal indexing cam mechanism.